MBMB 451b

Copyright 2000, E.C. Niederhoffer

Lecture #2

You can click on the link to listen to a RealAudio file of the day's lecture. The following description contains the material discussed and the source of the overheads used during the presentation.

Code:

ECN = Created by me

PDF = pdf file

S = Segel (Enzyme Kinetics)

VV = Voet & Voet (Biochemistry)

Lecture 2 (1/20/99) 73 minutes

Announcements

• Remember to check the web site for RealAudio lectures and other materials

Lecture #1 review

• Introduction to enzymes, historical perspective, substrate specificity, coenzymes, regulation of enzymatic activity, enzyme nomenclature
• Rates of enzymatic reactions

Rates of enzymatic reactions (PDF file for this topic)

• Chemical kinetics, elementary reactions, rates of reactions, transition state theory (PDF pp. 1, 2, 3, VV Figs 13-1, 13-2, 13-5, 13-6)
• Enzyme kinetics, Michaelis-Menten equation, analysis of kinetic data, reversible reactions (PDF p. 4, VV Fig 13-7, ECN, VV Figs 13-8, 13-9, S Figs II-9, II-10, II-11, VV Table 13-1, PDF p. 5)
• Inhibition, competitive inhibition, uncompetitive inhibition, mixed inhibition (S Fig III-1, VV Figs 13-10, 13-11, S Figs III-2, III-4, III-5, III-21, VV Fig 13-12, S Figs III-22, III-23, III-24, VV Fig 13-13, S Figs III-16, III-17, III-18)

Preview of lecture #3

• Rates of enzymatic reactions, mixed inhibition, effects of pH, bisubstrate reactions

Remember to look over the Study Guides and textbook sections for additional illustrations that may assist in your understanding of the presented material.

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