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Eric C. Niederhoffer, Ph.D. Associate Professor of Biochemistry and Molecular Biology Southern Illinois University School of Medicine 600 Agriculture Drive, Carbondale, IL 62901-6503 Rm 112 Lindegren, 618-453-6467 eniederhoffer@siumed.edu Copyright 2000- , E.C. Niederhoffer. All Rights Reserved. All trademarks and copyrights are the property of their respective owners.

Open (1ig8) and closed (1hkg) forms of hexokinase from Saccharomyces cerevisiae

The open form allows binding of glucopyranose and ATP while the closed form excludes water from the active site in preparation for the catalytic reaction.

• What structural elements are present in each molecule?
• What is the subunit composition?
• What gross comparisons can be made with the two structures?
• Which residues participate in the phosphorylation reaction?
• Which residues form a hydrophobic channel?

Kuser, P. R., S. Krauchenco, O. A. Antunes, and I. Polikarpov. 2000. The high resolution crystal structure of yeast hexokinase Pii with the correct primary sequence provides new insights into its mechanism of action. J. Biol. Chem. 275:20814-20821.

Steitz, T. A., M. Shoham, and W. S. Bennett Jr. 1981. Structural dynamics of yeast hexokinase during catalysis. Philos. Trans. R. Soc. Lond. B Biol. Sci. 293:43-52.

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