MBMB 570
Metallobiochemistry
Eric C. Niederhoffer, Ph.D.
- Associate Professor of Biochemistry and Molecular
Biology
- Southern Illinois University School of Medicine
- Rm 112 Lindegren, 453-6467, eniederhoffer@siumed.edu
Copyright 2001-
, E.C. Niederhoffer.
All Rights Reserved.
All trademarks and copyrights are the property of their respective
owners.
GENERAL
INFORMATION:
Metallobiochemistry (MBMB 570, two credit
hours) is intended to provide you with a foundation in modern
inorganic biochemistry with specific emphasis on metal-containing
proteins. We will draw upon knowledge of biochemistry, analytical,
inorganic, organic and physical chemistry, as well as common mathematical
approaches to problem solving. Familiarity with the internet and
either PC or Mac computers is required. Classes meet on Monday
and Wednesday afternoons from 3:00 to 3:50 p.m., unless otherwise
specified.
I reserve the right to change or modify
the syllabus.
RESOURCES:
We will use the following textbook and selected articles from the current literature:
You may find it useful to review fundamental
concepts as outlined in any of the recent biochemistry and inorganic
textbooks, including:
- Voet, D., J. G. Voet, and C. W.
Pratt. 1999. Fundamentals
of biochemistry, John Wiley & Sons,
Inc., New York. An upgrade edition of this should soon be available.
- Cotton, F. A., G. Wilkinson, C.
A. Murillo, and M. Bochmann.
1999. Advanced
inorganic chemistry, 6th ed. John Wiley
& Sons, Inc., New York.
We will use the molecular viewing applications
Protein
Explorer, RasMol and Swiss-PdbViewer during this course. See "Chime
Square" for additional information
concerning macromolecular viewing of protein and nucleic acid
structures.
GRADING POLICY:
Your performance in this course will
be based on examinations and problems sets. Examination and problem
set dates will be announced in class and posted to the course
web site. I reserve the right
to change the date of examinations and problem sets.
There will be no make-up examinations
or problem sets. A valid signed University-approved or medical
excuse must be presented to me as soon as possible if you miss
an examination. In general, only
medical emergencies are considered legitimate excuses. One examination
performance may be offset by the average of your second lowest
examination score and the final examination score.
COURSE OUTLINE: (Tentative Topics for Discussion)
Fundamentals of inorganic
biochemistry 4
lectures
- the elements
- formal oxidation states and coordination
geometries
- classification of metal ions and ligands
(HSAB theory)
- stability constants
- stabilization of oxidation states
- ligand field stabilization energy
- kinetics and mechanisms of reactions
involving metal complexes
- electron-transfer reactions
- reaction kinetics and thermodynamics
- biological ligands
Experimental methods 4 lectures
- introduction to spectroscopy
- optical spectroscopy
- magnetic resonance and related disciplines
- solution methods dependent on X-radiation
and g-radiation
- electrochemical methods
- enzyme kinetics
- measuring the molecular mass of a protein
- measurement of macromolecule-ligand
binding affinities
First Examination
- Fundamentals and experimental methods
Transport and storage 4 lectures
- metal ion uptake and transmembrane
ion transport, siderophores
- transport and storage of metal ions
in vivo, transferrin, ferritin, metallothionine
Metalloproteins and metalloenzymes:
(I) oxygen carriers and hydrolases
4 lectures
- oxygen carriers, myoglobin, hemoglobin,
hemerythrin, hemocyanin
- hydrolase enzymes, carboxypeptidase
A, alkaline phosphatase, purple acid phosphatase, arginase, urease
- hydro-lase enzymes, aconitase
Second Examination
- Transport & storage and metalloproteins & metalloenzymes
Metalloproteins and metalloenzymes:
(II) redox chemistry 3 lectures
- prosthetic centers, cofactors, and
coenzymes
- protein-protein electron transfer
Cell toxicity 2 lectures
- oxygen toxicity, superoxide dismutase
- metal toxicity
Case studies
3 lectures
- cytochrome c oxidase
- mercuric reductase
- nitrogenase
Third Examination
- Metalloproteins & metalloenzymes, cell toxicity and case
studies
Final Examination
- Semester topics
Biochemistry and Molecular Biology Home Page
For more information or comments about
this page contact:
eniederhoffer@siumed.edu